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Differences in cell type have a larger contribution to differing APA usage than age (a) Venn diagram showing the number of genes with differentially used APA sites in synovium and cartilage. Synovium contains more genes with APA changes, with 17 genes shared between both tissues. (b) Table listing these 17 genes with differential APA site usage detected in both synovium and cartilage. Only <t>NRG1</t> shows consistent polyadenylation site usage across both tissues. (c, d) Normalised APA proportion matrices of the NRG1 in (c) synovium and (d) cartilage. Usage of the intronic APA site at 27 12857304 12857423 + is annotated in blue. n = 5-6. (e) qPCR assay strategy: Primer pair 1 was designed to determine whether the intronic APA site at 27 12857304 12857423 + is used to transcribe a small non-coding RNA. Primer pair 2 was designed to assess whether the usage of the same APA site results in a truncated mRNA. (f) qPCR analysis of NRG1 using primer pair 1 was performed on synovium and cartilage samples from young and old horses. n = 5. Statistical significance was determined using an unpaired t-test, with p < 0.05 considered significant.
Anti Nrg1 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti nrg1 antibody/product/Proteintech
Average 94 stars, based on 1 article reviews
anti nrg1 antibody - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
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Proteintech anti nrg1
Differences in cell type have a larger contribution to differing APA usage than age (a) Venn diagram showing the number of genes with differentially used APA sites in synovium and cartilage. Synovium contains more genes with APA changes, with 17 genes shared between both tissues. (b) Table listing these 17 genes with differential APA site usage detected in both synovium and cartilage. Only <t>NRG1</t> shows consistent polyadenylation site usage across both tissues. (c, d) Normalised APA proportion matrices of the NRG1 in (c) synovium and (d) cartilage. Usage of the intronic APA site at 27 12857304 12857423 + is annotated in blue. n = 5-6. (e) qPCR assay strategy: Primer pair 1 was designed to determine whether the intronic APA site at 27 12857304 12857423 + is used to transcribe a small non-coding RNA. Primer pair 2 was designed to assess whether the usage of the same APA site results in a truncated mRNA. (f) qPCR analysis of NRG1 using primer pair 1 was performed on synovium and cartilage samples from young and old horses. n = 5. Statistical significance was determined using an unpaired t-test, with p < 0.05 considered significant.
Anti Nrg1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti nrg1/product/Proteintech
Average 94 stars, based on 1 article reviews
anti nrg1 - by Bioz Stars, 2026-02
94/100 stars
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Differences in cell type have a larger contribution to differing APA usage than age (a) Venn diagram showing the number of genes with differentially used APA sites in synovium and cartilage. Synovium contains more genes with APA changes, with 17 genes shared between both tissues. (b) Table listing these 17 genes with differential APA site usage detected in both synovium and cartilage. Only <t>NRG1</t> shows consistent polyadenylation site usage across both tissues. (c, d) Normalised APA proportion matrices of the NRG1 in (c) synovium and (d) cartilage. Usage of the intronic APA site at 27 12857304 12857423 + is annotated in blue. n = 5-6. (e) qPCR assay strategy: Primer pair 1 was designed to determine whether the intronic APA site at 27 12857304 12857423 + is used to transcribe a small non-coding RNA. Primer pair 2 was designed to assess whether the usage of the same APA site results in a truncated mRNA. (f) qPCR analysis of NRG1 using primer pair 1 was performed on synovium and cartilage samples from young and old horses. n = 5. Statistical significance was determined using an unpaired t-test, with p < 0.05 considered significant.
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Differences in cell type have a larger contribution to differing APA usage than age (a) Venn diagram showing the number of genes with differentially used APA sites in synovium and cartilage. Synovium contains more genes with APA changes, with 17 genes shared between both tissues. (b) Table listing these 17 genes with differential APA site usage detected in both synovium and cartilage. Only NRG1 shows consistent polyadenylation site usage across both tissues. (c, d) Normalised APA proportion matrices of the NRG1 in (c) synovium and (d) cartilage. Usage of the intronic APA site at 27 12857304 12857423 + is annotated in blue. n = 5-6. (e) qPCR assay strategy: Primer pair 1 was designed to determine whether the intronic APA site at 27 12857304 12857423 + is used to transcribe a small non-coding RNA. Primer pair 2 was designed to assess whether the usage of the same APA site results in a truncated mRNA. (f) qPCR analysis of NRG1 using primer pair 1 was performed on synovium and cartilage samples from young and old horses. n = 5. Statistical significance was determined using an unpaired t-test, with p < 0.05 considered significant.

Journal: bioRxiv

Article Title: Characterising the effect of age and sex on post-transcriptional regulation in synovial joint tissues

doi: 10.1101/2025.09.18.677035

Figure Lengend Snippet: Differences in cell type have a larger contribution to differing APA usage than age (a) Venn diagram showing the number of genes with differentially used APA sites in synovium and cartilage. Synovium contains more genes with APA changes, with 17 genes shared between both tissues. (b) Table listing these 17 genes with differential APA site usage detected in both synovium and cartilage. Only NRG1 shows consistent polyadenylation site usage across both tissues. (c, d) Normalised APA proportion matrices of the NRG1 in (c) synovium and (d) cartilage. Usage of the intronic APA site at 27 12857304 12857423 + is annotated in blue. n = 5-6. (e) qPCR assay strategy: Primer pair 1 was designed to determine whether the intronic APA site at 27 12857304 12857423 + is used to transcribe a small non-coding RNA. Primer pair 2 was designed to assess whether the usage of the same APA site results in a truncated mRNA. (f) qPCR analysis of NRG1 using primer pair 1 was performed on synovium and cartilage samples from young and old horses. n = 5. Statistical significance was determined using an unpaired t-test, with p < 0.05 considered significant.

Article Snippet: The membrane was incubated overnight at 4°C with anti-NRG1 antibody (1:600, Proteintech, Rosemont, IL, USA, #66492-1), followed by incubation with a secondary anti-mouse LI-COR antibody at RT.

Techniques: